Making DNA chips, Stephen Fodor
Interviewee: Stephen Fodor. Stephen Fodor talks about the photolithographic technique used to synthesize pieces of DNA on the surface of GeneChipsâ„¢. (DNAi Location: Manipulation > Techniques > Large-scale analysis > Interviews > GeneChipsâ„¢, step by step)
The way we make these DNA chips is that we actually build the DNA step by step at each location on one of these chips. In a process known as photolithography we excite with light a certain region on the chip and that prepares that particular site for chemical reaction. One of the four building blocks, A, C, G or T are applied and wherever we shine the light, that building block will stick and grow. And so we go through a multiple number of steps in this way, over literally millions of different sites on a large piece of glass, and what we get are literally millions of different precise locations on the chip, each with their own different strand of DNA on it.
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Stephen Fodor continues his discussion of the experiments that laid the groundwork for GeneChip technology.
Stephen Fodor talks about bringing the knowledge gathered by the Human Genome Project to the individual researcher.
Stephen Fodor discusses the experiments that laid the groundwork for GeneChip technology.
Stephen Fodor talks about manufacturing the gene chip.
Stephen Fodor talks about limit to the gene chip.
Stephen Fodor is Chairman and CEO of Affymetrix, Inc
The cycles of the polymerase chain reaction (PCR).
In the early 1990s, Stephen Fodor and his team developed a technique to produce miniature arrays of biological molecules
Stephen Fodor talks about how an individual's gene expression profile can help determine what therapies might work best.
Igor Dawid and Thomas Sargent explain how they developed subtractive mRNA hybrization to find genes expressed by different cell types. Pat Brown and Steve Fodor show how genomes can be screened with DNA arrays and GeneChips™