Challenge of isolating a gene, Stanley Cohen
Interviewee: Stanley Cohen. Stanley Cohen talks about the challenge of isolating and studying individual genes. (DNAi Location: Manipulation > Revolution > players > Herbert Boyer and Stanley Cohen > One of the great challenges of biology)
One of the challenges of biology has been to isolate and study individual genes. If you can imagine a mammalian genome with tens of thousands of genes in it, and the task of really understanding how each of them works is, is a very monumental task. But if those genes can be isolated and studied individually, if they can be expressed individually and the consequences of that expression analyzed, then one begins to get a handle on understanding the way that complex organisms work and how they develop.
herbert boyer,stanley cohen,mammalian genome,gene expression,dnai,monumental task,interviewee,genes,organisms,manipulation,biology,tens of thousands,consequences,challenges
- ID: 15075
- Source: DNALC.DNAi
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15074. Taking apart plasmid DNA, Stanley Cohen
Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
15028. Interest in plasmids, Herbert Boyer
Herb Boyer talks about Stanley Cohen's and his interest in plasmids as vectors for DNA.
16033. Stanley Cohen and Herbert Boyer, 1972
Genes can be moved between species.
15030. Implications of recombinant DNA work, Herbert Boyer
Herb Boyer reflects on the importance of their work on rDNA technology and its impact on understanding the genetics of higher organisms.
15915. The first recombinant DNA
Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA.
15916. DNA transformation
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
15034. The initial investment in Genentech, Herbert Boyere
Herb Boyer reflects on his initial investment in Genentech.
15025. On the phenomenon of restriction, Paul Berg
Paul Berg speaks about Herbert Boyer's research into the process by which an organism, such as a bacterium, can recognize and destroy foreign DNA.
15087. Genentech's approach to making insulin, David Goeddel
David Goeddel was one of the first scientists Herb Boyer hired for the insulin project. Here, David Goeddel talks about Genentech's general strategy for synthesizing insulin.