Challenge of isolating a gene, Stanley Cohen
Interviewee: Stanley Cohen. Stanley Cohen talks about the challenge of isolating and studying individual genes. (DNAi Location: Manipulation > Revolution > players > Herbert Boyer and Stanley Cohen > One of the great challenges of biology)
One of the challenges of biology has been to isolate and study individual genes. If you can imagine a mammalian genome with tens of thousands of genes in it, and the task of really understanding how each of them works is, is a very monumental task. But if those genes can be isolated and studied individually, if they can be expressed individually and the consequences of that expression analyzed, then one begins to get a handle on understanding the way that complex organisms work and how they develop.
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Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
Herb Boyer talks about Stanley Cohen's and his interest in plasmids as vectors for DNA.
Genes can be moved between species.
Herb Boyer reflects on the importance of their work on rDNA technology and its impact on understanding the genetics of higher organisms.
Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA.
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
Herb Boyer reflects on his initial investment in Genentech.
Paul Berg speaks about Herbert Boyer's research into the process by which an organism, such as a bacterium, can recognize and destroy foreign DNA.
David Goeddel was one of the first scientists Herb Boyer hired for the insulin project. Here, David Goeddel talks about Genentech's general strategy for synthesizing insulin.