On the phenomenon of restriction, Paul Berg
Interviewee: Paul Berg. Paul Berg speaks about Herbert Boyer's research into the process by which an organism, such as a bacterium, can recognize and destroy foreign DNA. (DNAi Location: Manipulation > Techniques > Cutting & Pasting > Interviews > On the phenomenon of restriction)
Boyer, Herb Boyer had been studying restriction, the phenomenon of restriction. And he, along with a few others, realized and discovered that restriction, restriction was defined as if you took DNA from one organism and put it into another they destroyed it. But if you took their own DNA and put it in, it went in and survived, so there, there had to be some way in which a given organism recognized its own DNA and also recognized somebody else's DNA as being foreign and destroyed it.
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Paul Berg discusses the usefulness of recombinant DNA to isolate and study genes.
Paul Berg speaks about his student Janet Mertz's experiment to make the first recombinant DNA molecule.
Paul Berg's student, Janet Mertz, planned an experiment that would recombine DNA from a monkey virus with DNA from a bacterium that lives in the human gut. Berg describes colleague Bob Pollack's outrage at this.
Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
Herb Boyer reflects on the importance of their work on rDNA technology and its impact on understanding the genetics of higher organisms.
Paul Berg talks about why experiments with recombinant DNA set off a firestorm of controversy, including a moratorium on further experimentation with rDNA.
Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA.
Paul Berg talks about possible dangers of recombinant DNA.
Paul Berg recollects his reaction to his colleague Bob Pollack's opposition to experimentation with recombinant DNA.
Herb Boyer talks about Stanley Cohen's and his interest in plasmids as vectors for DNA.