Interest in plasmids, Herbert Boyer
Interviewee: Herbert Boyer. Herb Boyer talks about Stanley Cohen's and his interest in plasmids as vectors for DNA. (DNAi Location: Manipulation > Revolution > Players > Herbert Boyer and Stanley Cohen > Interest in plasmids)
We both had an interest in plasmids, these are small mini-chromosomes, usually non-essential for bacterial existence. But they had a strong medical importance because these plasmids carried genes that gave bacteria resistance to antibiotics, so they have a big medical importance, and Stanley had an interest in them. Also because they were small pieces of DNA they could be isolated and some of their properties could be manipulated and you could do experiments with them.
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Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
Herb Boyer reflects on the importance of their work on rDNA technology and its impact on understanding the genetics of higher organisms.
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
Stan Cohen and Herb Boyer "invented" recombinant DNA technology.
Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA.
Genes can be moved between species.
Stanley Cohen talks about the challenge of isolating and studying individual genes.
Herbert Boyer: Former varsity lineman turned biotech bigwig. Expert at cutting DNA before most people knew it could be done. Stanley Cohen: A born tinkerer; figured out the trick of using loops of DNA called plasmids to transform bacterial DNA