Virtual Lab Experiments in Biotechnology: Bacterial Transformation
Cold Spring Harbor Laboratory's DNA Learning Center presented this course as a service to help engage teachers and students in China during the coronavirus school closures. The bacterial transformation experiment illustrates the direct link between an organism's genetic complement (genotype) and its observable characteristics (phenotype). A gene for antibiotic resistance is introduced into the bacterium E. coli. Following overnight incubation, transformed bacteria are compared to unexposed bacteria for their ability to grow in the presence of ampicillin.
e coli bacteria,dna transformation,dna molecule,herbert boyer,stanley cohen,recombinant dna,dna sequence,e coli,e. coli,plasmid,expression
- ID: 17054
- Source: DNALC
- Download: MP4 Video
15916. DNA transformation
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
16705. Animation 34: Genes can be moved between species.
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
16721. Biography 34: Herb W. Boyer (1936 - )
Herb Boyer and Stan Cohen "invented" recombinant DNA technology.
15074. Taking apart plasmid DNA, Stanley Cohen
Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is
15162. Preparing the DNA for sequencing, Shane Yeager
Shane Yeager, from the Whitehead Institute Center for Genome Research, explains the processes of storing and preparing DNA for sequencing.
15028. Interest in plasmids, Herbert Boyer
Herb Boyer talks about Stanley Cohen's and his interest in plasmids as vectors for DNA.
15915. The first recombinant DNA
Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA.
15020. Possible dangers of recombinant DNA, Paul Berg
Paul Berg talks about possible dangers of recombinant DNA.
15626. Paul Berg and Stanley Cohen
Herbert Boyer: Former varsity lineman turned biotech bigwig. Expert at cutting DNA before most people knew it could be done. Stanley Cohen: A born tinkerer; figured out the trick of using loops of DNA called plasmids to transform bacterial DNA