Virtual Lab Experiments in Biotechnology: Bacterial Transformation
Cold Spring Harbor Laboratory's DNA Learning Center presented this course as a service to help engage teachers and students in China during the coronavirus school closures. The bacterial transformation experiment illustrates the direct link between an organism's genetic complement (genotype) and its observable characteristics (phenotype). A gene for antibiotic resistance is introduced into the bacterium E. coli. Following overnight incubation, transformed bacteria are compared to unexposed bacteria for their ability to grow in the presence of ampicillin.
e coli bacteria,dna transformation,dna molecule,herbert boyer,stanley cohen,recombinant dna,dna sequence,e coli,e. coli,plasmid,expression
- ID: 17054
- Source: DNALC
- Download: MP4 Video
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
Herb Boyer and Stan Cohen "invented" recombinant DNA technology.
Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is
Shane Yeager, from the Whitehead Institute Center for Genome Research, explains the processes of storing and preparing DNA for sequencing.
Herb Boyer talks about Stanley Cohen's and his interest in plasmids as vectors for DNA.
Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA.
Paul Berg talks about possible dangers of recombinant DNA.
Herbert Boyer: Former varsity lineman turned biotech bigwig. Expert at cutting DNA before most people knew it could be done. Stanley Cohen: A born tinkerer; figured out the trick of using loops of DNA called plasmids to transform bacterial DNA