Transformation

DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is widely used today to obtain high-efficiency transforming cells.

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  • ID: 15918
  • Source: DNALC.DNAi

Related Content

16705. Animation 34: Genes can be moved between species.

Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.

  • ID: 16705
  • Source: DNALC.DNAFTB

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Improving on the Mandel and Higa method of DNA transformation.

  • ID: 16715
  • Source: DNAFTB

15916. DNA transformation

Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."

  • ID: 15916
  • Source: DNAi

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  • ID: 16395
  • Source: DNALC.DNAFTB

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Shane Yeager, from the Whitehead Institute Center for Genome Research, explains the processes of storing and preparing DNA for sequencing.

  • ID: 15162
  • Source: DNAi

16687. Concept 33: Genes can be turned on and off.

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  • ID: 16687
  • Source: DNAFTB

16657. Some DNA does not encode protein.

DNAFTB Animation 31: Roy Britten presents his work with David Kohne on repetitive DNA and its evolutionary origins.

  • ID: 16657
  • Source: DNALC.DNAFTB

16720. Biography 34: Stan Norman Cohen (1935 - )

Stan Cohen and Herb Boyer "invented" recombinant DNA technology.

  • ID: 16720
  • Source: DNAFTB

16613. Animation 28: Some types of mutations are automatically repaired.

Stan Rupert explains UV damage and light-activated DNA repair systems and Richard Setlow's work on thymine dimer repair.

  • ID: 16613
  • Source: DNALC.DNAFTB