DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is widely used today to obtain high-efficiency transforming cells.
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- ID: 15918
- Source: DNALC.DNAi
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
Improving on the Mandel and Higa method of DNA transformation.
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
Joshua Lederberg worked with bacterial genetics while Alfred Hershey showed that DNA is responsible for the reproduction of new viruses in a cell.
Shane Yeager, from the Whitehead Institute Center for Genome Research, explains the processes of storing and preparing DNA for sequencing.
Organisms can regulate gene expression.
DNAFTB Animation 31: Roy Britten presents his work with David Kohne on repetitive DNA and its evolutionary origins.
Stan Cohen and Herb Boyer "invented" recombinant DNA technology.
Stan Rupert explains UV damage and light-activated DNA repair systems and Richard Setlow's work on thymine dimer repair.