Video 34: Douglas Hanahan, clip 1
Doug Hanahan is a professor of biochemistry at UCSF. He refined transformation techniques, and developed the current theories on the mechanisms of DNA uptake.
douglas hanahan, dna transformation, dna uptake, transformation techniques, mandel, biochemistry mechanisms
- ID: 16715
- Source: DNALC.DNAFTB
- Download: MPEG 4 Video
16719. Video 34: Douglas Hanahan, clip 5
Size of DNA and transformation efficiencies.
16716. Video 34: Douglas Hanahan, clip 2
The problem of getting DNA into a bacteria.
16717. Video 34: Douglas Hanahan, clip 3
How big are those bacterial pores?
16718. Video 34: Douglas Hanahan, clip 4
Definitions of "cloning."
16722. Biography 34: Doug Hanahan (1951 - )
Doug Hanahan refined transformation methods for DNA uptake into bacteria.
DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is
15916. DNA transformation
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
16705. Animation 34: Genes can be moved between species.
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
15074. Taking apart plasmid DNA, Stanley Cohen
Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.
16393. Problem 17: A gene is made of DNA.
Experiment with rough and smooth Pneumococcus DNA.