Video 34: Douglas Hanahan, clip 1

Doug Hanahan is a professor of biochemistry at UCSF. He refined transformation techniques, and developed the current theories on the mechanisms of DNA uptake.

douglas hanahan, dna transformation, dna uptake, transformation techniques, mandel, biochemistry mechanisms

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16719. Video 34: Douglas Hanahan, clip 5

Size of DNA and transformation efficiencies.

  • ID: 16719
  • Source: DNAFTB

16716. Video 34: Douglas Hanahan, clip 2

The problem of getting DNA into a bacteria.

  • ID: 16716
  • Source: DNAFTB

16717. Video 34: Douglas Hanahan, clip 3

How big are those bacterial pores?

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16718. Video 34: Douglas Hanahan, clip 4

Definitions of "cloning."

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  • Source: DNAFTB

16722. Biography 34: Doug Hanahan (1951 - )

Doug Hanahan refined transformation methods for DNA uptake into bacteria.

  • ID: 16722
  • Source: DNAFTB

15918. Transformation

DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is

  • ID: 15918
  • Source: DNAi

15916. DNA transformation

Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."

  • ID: 15916
  • Source: DNAi

16705. Animation 34: Genes can be moved between species.

Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.

  • ID: 16705
  • Source: DNALC.DNAFTB

15074. Taking apart plasmid DNA, Stanley Cohen

Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.

  • ID: 15074
  • Source: DNAi

16393. Problem 17: A gene is made of DNA.

Experiment with rough and smooth Pneumococcus DNA.

  • ID: 16393
  • Source: DNALC.DNAFTB