Problem 17: A gene is made of DNA.
Experiment with rough and smooth Pneumococcus DNA.
HI! Avery and his group used S-strain DNA to transform rough-coat (R) strain to the smooth-coat (S) strain. You do a similar experiment except you use R DNA instead of S DNA. What happens? Would the R strain be transformed to the S strain? DNA is DNA, transformation will take place. (No, R to S transformation requires a special donor DNA.) R strain DNA will transform R to S, but at a much lower frequency. (No, R strain DNA cannot transform R to S.) R strain DNA canâ€™t transform R to S. (That is correct) R strain DNA will transform R to S, but the new S will not be infectious. (No, there is no "new" S in this case.) The R strain is missing the gene that makes the sugar coat needed for virulence. So adding DNA from the R strain will not transform R to S. When Avery and his group isolated DNA, they noticed that the DNA solution was very viscous, thick, and stringy. When they incubated the DNA solution with DNase, the viscosity disappeared. The same result occurs if the DNA sample is vortexed. If you do an Avery transformation experiment using S-strain DNA that has been vortexed for a long time, it loses its transformation ability. Why? Vortexing breaks the DNA into small pieces. The genes are no longer intact. (That is correct) Vortexing mixes the DNA, and makes new DNA that doesnâ€™t have the transforming ability. (No, vortexing merley breaks up the DNA, it does not make new DNA.) Itâ€™s not DNA after all that causes transformation. (No, DNA is the transforming principle.) Vortexing breaks the DNA backbone. As a result, large DNA molecules are broken into short pieces. It is unlikely that any of these short DNA pieces are long enough to contain an entire gene. You figure out a way to break the S strain's DNA molecule exactly in half, then separate the two halves. You then use each half in a transformation experiment. Half#2 doesnâ€™t have the gene that transforms R to S. (That is correct.) Half#1 has all the genes, Half#2 is junk DNA. (No, you donâ€™t know that Half#2 is junk DNA. You only know that it doesnâ€™t have the transforming ability.) Half#1 worked this time, in another experiment Half#2 will work. (No, if Half#2 canâ€™t transform, it doesnâ€™t matter how many times you try.) Half#1 transforms the R strain to S. This means that the gene for transformation is somewhere on that piece of DNA. Half#2 canâ€™t transform because it doesnâ€™t have the gene. CONGRATULATIONS!!! YOU'RE SO SMART!
dna transformation, dna molecules, rough coat, smooth coat, pneumococcus, sugar coat, transforming principle, virulence, avery
- ID: 16393
- Source: DNALC.DNAFTB
Oswald Avery explains Fred Griffith's and his own work with Pneumococcus bacteria.
Oswald Avery's team proves that DNA, not protein, is the genetic molecule.
Oswald Avery, circa 1930.
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
A page from the May 15, 1943 letter from Oswald Avery to his brother Roy. In the letter Avery speculated on how transformation could happen. Avery never publicly connected genes with DNA and his transformation experiments.
Joshua Lederberg worked with bacterial genetics while Alfred Hershey showed that DNA is responsible for the reproduction of new viruses in a cell.
How the bacterial transformation experiments provided the first real opportunity to study the chemical nature of the gene.
In 1944, Maclyn McCarty and his colleagues, Colin MacLeod and Oswald Avery published their landmark paper on the transforming ability of DNA.
Describing the in vitro transformation experiments: the effect of removing polysaccharides from the bacterial extracts.
Describing the in vitro transformation experiments: the effect of destroying nucleic acids.