Video 34: Douglas Hanahan, clip 2
Doug Hanahan is a professor of biochemistry at UCSF. He refined transformation techniques, and developed the current theories on the mechanisms of DNA uptake.
douglas hanahan, dna uptake, transformation techniques, biochemistry, bacteria mechanisms
- ID: 16716
- Source: DNALC.DNAFTB
- Download: MPEG 4 Video
Improving on the Mandel and Higa method of DNA transformation.
Size of DNA and transformation efficiencies.
How big are those bacterial pores?
Definitions of "cloning."
Doug Hanahan refined transformation methods for DNA uptake into bacteria.
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
Molecular geneticist Douglas Wallace talks about the origin of our mitochondria and the DNA within.
DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is