Video 34: Douglas Hanahan, clip 2

Doug Hanahan is a professor of biochemistry at UCSF. He refined transformation techniques, and developed the current theories on the mechanisms of DNA uptake.

douglas hanahan, dna uptake, transformation techniques, biochemistry, bacteria mechanisms

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16715. Video 34: Douglas Hanahan, clip 1

Improving on the Mandel and Higa method of DNA transformation.

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16719. Video 34: Douglas Hanahan, clip 5

Size of DNA and transformation efficiencies.

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16717. Video 34: Douglas Hanahan, clip 3

How big are those bacterial pores?

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16718. Video 34: Douglas Hanahan, clip 4

Definitions of "cloning."

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16722. Biography 34: Doug Hanahan (1951 - )

Doug Hanahan refined transformation methods for DNA uptake into bacteria.

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Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."

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16705. Animation 34: Genes can be moved between species.

Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.

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15918. Transformation

DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is

  • ID: 15918
  • Source: DNAi