Video 34: Douglas Hanahan, clip 5
Doug Hanahan is a professor of biochemistry at UCSF. He refined transformation techniques, and developed the current theories on the mechanisms of DNA uptake.
mechanisms, biochemistry, ucsf, efficiencies, transformation techniques, douglas hanahan, dna uptake
- ID: 16719
- Source: DNALC.DNAFTB
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Improving on the Mandel and Higa method of DNA transformation.
The problem of getting DNA into a bacteria.
How big are those bacterial pores?
Definitions of "cloning."
Doug Hanahan refined transformation methods for DNA uptake into bacteria.
Stanley Cohen and Herbert Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."
DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
Professor Douglas Hanahan discusses how cancer acquires capabilities and these capabilities are all, to some approximation, necessary to produce a successful tumor.
Professor Douglas Hanahan explains that a fundamental property of multi-cellular organisms is the capability to have cells commit suicide or undergo apoptosis, which is a form of programmed cell death.