Technique to control a gene (Part 2), Mario Capecchi
Interviewee: Mario Capecchi. Mario Capecchi continues his explanation of the technique he uses to control genes in mice. (DNAi Location: Applications > Genes and medicine > Gene targeting > Mario Capecchi > Technique to control a gene-Part II)
And this enzyme recognizes those sequences and then does a cross-over, what it does is cut and, and re-paste across this junction and in the process the DNA is going from here to here to here, and then the process deletes the gene. So if we control where Cre is expressed in the, where it's functional anywhere in the mouse, for example just in the limb bud, then this gene will only be excised from the cells in the limb bud and result essentially in DNA in which the gene is completely gone.
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Mario Capecchi explains the technique he uses to control genes in mice.
Mario Capecchi discusses the similarities between mouse and human limb formation, and how his work might lead to limb regeneration in the future.
Mario Capecchi discusses homologous recombination, the technique he developed to introduce a desired mutation into the DNA of living cells.
Mario Capecchi talks about manipulating embryonic stem (ES) cells to make specific mutations in mouse embryos.
Mario Capecchi talks about the possibility of introducing an artificial chromosome into cells to slow the aging process.
Mario Capecchi talks about the possible use of embryonic stem cells and gene targeting techniques to develop new therapies for for diabetes and Parkinson's.
Mario Capecchi talks about the advantages of working with mice to study genetic disorders.
Mario Capecchi talks about the possibility of correcting genetic defects.
Mario Capecchi discusses the idea that someday therapies may be created to correct defective genes in egg and sperm cells.
Mario Capecchi talks about Switching genes off.