Homologous recombination, Mario Capecchi
Interviewee: Mario Capecchi. Mario Capecchi discusses homologous recombination, the technique he developed to introduce a desired mutation into the DNA of living cells. (DNAi Location: Applications > Genes and medicine > Gene targeting > Mario Capecchi > Homologous recombination)
In this vial we have embryo-derived stem cells, these are pluripotent cells which we can keep in a state of limbo. They're normally capable of making all different tissues, but when they're in isolation they simply stay as undifferentiated cells. We add to this vial also the DNA that we want to modify that has the gene modification, then we introduce, we put electric probes into that solution and then we push a button and that gives rise essentially to a voltage across those cells, opens up little holes in the cells and the DNA slips inside and then participates in what's called homologous recombination to introduce the mutation we created in the test tube to the genome of the living cells. Then by taking those cells and introducing them into an embryo, then they can actually make a mouse embryo and thereby introduce that mutation we created in the test tube to the genome of the living mouse.
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Mario Capecchi talks about manipulating embryonic stem (ES) cells to make specific mutations in mouse embryos.
This method uses homologous recombination to disable a gene of interest to produce a genetic knockout.
Mario Capecchi describes proteomics; the large-scale study of protein structure and function. Brian Sauer explains gene knock outs.
Mario Capecchi talks about the possible use of embryonic stem cells and gene targeting techniques to develop new therapies for for diabetes and Parkinson's.
Mario Capecchi explains the technique he uses to control genes in mice.
Mario Capecchi continues his explanation of the technique he uses to control genes in mice.
Mario Capecchi talks about the possibility of introducing an artificial chromosome into cells to slow the aging process.
Mice are small, easy to keep, and complete a generation in only ten weeks. They are also rather closely related to human beings.
Model organisms share with humans many key biochemical and physiological functions that have been conserved (maintained) by evolution.
Experiment with gene knock outs.