Gene targeting, Mario Capecchi
Interviewee: Mario Capecchi. Mario Capecchi talks about manipulating embryonic stem (ES) cells to make specific mutations in mouse embryos. (DNAi Location: Applications > Genes and medicine > Gene targeting > Mario Capecchi > Gene targeting)
This is a plate of ES cells and they're growing on the surface and under the microscope we can see them. And it's these cells that we actually manipulate, that is which manipulate their genome and make specific mutations using a technique called gene targeting, which allows us to modify a particular gene. And once we've done that, then we can take these cells and then introduce them back into an early embryo of a mouse, which is called the blastocyst, re-implant that into the uterus and that then grows into a mouse and then that gene that we modified in these cells will then be modified in every cell of the embryo.
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Mario Capecchi discusses homologous recombination, the technique he developed to introduce a desired mutation into the DNA of living cells.
Mario Capecchi talks about the possible use of embryonic stem cells and gene targeting techniques to develop new therapies for for diabetes and Parkinson's.
Mario Capecchi explains the technique he uses to control genes in mice.
Mario Capecchi continues his explanation of the technique he uses to control genes in mice.
Mario Capecchi talks about the possibility of introducing an artificial chromosome into cells to slow the aging process.
Mario Capecchi describes proteomics; the large-scale study of protein structure and function. Brian Sauer explains gene knock outs.
Mario Capecchi discusses the similarities between mouse and human limb formation, and how his work might lead to limb regeneration in the future.
Mario Capecchi talks about the advantages of working with mice to study genetic disorders.
Mario Capecchi talks about the possibility of correcting genetic defects.
Mario Capecchi discusses the idea that someday therapies may be created to correct defective genes in egg and sperm cells.