Technique to control a gene (Part I), Mario Capecchi
Interviewee: Mario Capecchi. Mario Capecchi explains the technique he uses to control genes in mice. (DNAi Location: Applications > Genes and medicine > Gene targeting > Mario Capecchi > Technique to control a gene-Part I)
So in order to be able to control a gene, not only inactivate it in every cell but to be able to control the gene in specific cells at a particular time in a mouse, a technique that is used is to combine essentially homologous recombination with what's called site-specific recombination. These are molecular scissors, what, a site-specific recombination recognizes so this is our gene of interest and we flank it by homologous recombination gene targeting with two sequences which are called loxP. And if we now, and if we introduce into this cell an enzyme called Cre, which is the site-specific recombination, what happens is these sequences line up. So here we have the DNA and it goes around like that, and here are the loxP sites.
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Mario Capecchi continues his explanation of the technique he uses to control genes in mice.
Mario Capecchi discusses homologous recombination, the technique he developed to introduce a desired mutation into the DNA of living cells.
Mario Capecchi talks about manipulating embryonic stem (ES) cells to make specific mutations in mouse embryos.
Mario Capecchi talks about the possibility of introducing an artificial chromosome into cells to slow the aging process.
Mario Capecchi talks about the possible use of embryonic stem cells and gene targeting techniques to develop new therapies for for diabetes and Parkinson's.
Mario Capecchi talks about the advantages of working with mice to study genetic disorders.
Mario Capecchi talks about the possibility of correcting genetic defects.
Mario Capecchi discusses the idea that someday therapies may be created to correct defective genes in egg and sperm cells.
Mario Capecchi discusses the similarities between mouse and human limb formation, and how his work might lead to limb regeneration in the future.
Mario Capecchi describes proteomics; the large-scale study of protein structure and function. Brian Sauer explains gene knock outs.