Making a DNA microarray, Patrick Brown

Interviewee: Pat Brown. Pat Brown discusses the early technology behind the microarray. (DNAi Location: Applications > Genes and medicine > genetic profiling > Patrick Brown > Making a microarray)

We built a very simple robot that used a very simple and ancient technology, the technology of the fountain pen, and what the robot does is it takes a set of fountain pens and dips them in each of these little ink wells in this 96-well plate, and the ink in these ink wells is the DNA that represents a human gene. Each well represents a different human gene. The pens dip into the wells and then they move over and print the DNA ink in a nice array on a little microscope slide like this and they repeat that process over and over again from hundreds of these plates, finally producing an array that represents about thirty thousand different human genes. And then we can use these microarrays in a very simple experimental procedure that allows us, that basically causes the spots to light up whenever the genome in a particular cell or tissue sample uses one of these genes, one of these words, in the script that it's writing.

dna microarray,microscope slide,human genes,genetic profiling,fountain pens,ink wells,location applications,early technology,pat brown,well plate,dnai,experimental procedure,gene expression,human gene,ancient technology,interviewee,microarrays,fountain pen,dips,genome

Related Content

15036. Why we developed the microarray, Patrick Brown

Pat Brown talks about developing microarray technology for genome-wide analysis.

  • ID: 15038
  • Type: video
  • Source: DNAi

15039. What's on a microarray, Patrick Brown

Pat Brown talks about how the 30,000 spots on the microarray represent genes.

  • ID: 15038
  • Type: video
  • Source: DNAi

15040. Using DNA microarrays to study cancer, Patrick Brown

Pat Brown talks about using microarrays to discover the differences between cancer cells and healthy cells.

  • ID: 15038
  • Type: video
  • Source: DNAi

15049. GeneChip™ technology, Stephen Fodor

Stephen Fodor talks about bringing the knowledge gathered by the Human Genome Project to the individual researcher.

  • ID: 15038
  • Type: video
  • Source: DNAi

15542. DNA microarray output

DNA microarrays provide the means to analyze patterns of gene expression at different timepoints in a living cell.

  • ID: 15038
  • Type: video
  • Source: DNAi

15037. The genome is the script, Patrick Brown

Pat Brown draws an analogy between the genome and a script that tells a cell how to behave.

  • ID: 15038
  • Type: video
  • Source: DNAi

15992. DNA microarrays

DNA microarrays provide the means to analyze patterns of gene expression at different timepoints in a living cell.

  • ID: 15038
  • Type: video
  • Source: DNAi

16756. Biography 36: Patrick Henry Brown (1954- )

Pat Brown changed the way genomes can be screened with DNA arrays.

  • ID: 15038
  • Type: video
  • Source: DNAFTB

15527. DNA microarray

DNA microarray

  • ID: 15038
  • Type: video
  • Source: DNAi

15920. DNA arrays

In the 1990s, DNA arrays provided the means to analyze patterns of gene expression at different timepoints in a living cell.

  • ID: 15038
  • Type: video
  • Source: DNAi