Genes get shuffled when chromosomes exchange pieces.
Alfred Sturtevant describes gene mapping.
I'm Alfred Sturtevant. I was a graduate student in T. H. Morgan's lab, and in 1913, I published the world's first genetic map as part of my Ph.D. thesis. The idea of mapping genes came from a discussion I had with my "boss" about the work of Belgian cytologist F. A. Janssens. He found that, early in meiosis, homologous chromosomes intertwine and exchange pieces. This process later became known as "crossing over." [ELECTRON MICROGRAPH OF CHROMOSOMAL CROSSOVER] To help visualize crossing over, let's colorize this micrograph and label parts of these homologous chromosomes. This is considered a double-crossover: two points of the chromatids overlap, and the section between the crossover points is exchanged. [CENTROMERE CHROMATIDS CROSSOVER SITE] My boss realized that a crossing over event can recombine alleles between homologous chromosomes. He used this ball diagram to illustrate what he meant. [DIAGRAM ADAPTED FROM T. H. MORGAN'S ORIGINAL] When a crossover happens, alleles that are far apart have a greater chance of being recombined. Alleles that are closer have a lower chance of being recombined. I came up with the idea of using recombinant data to construct a map of genes on the X chromosome. I mapped genes for three recessive traits – yellow body (y), white eyes (w), and miniature wings (m). First I made heterozygous female strains, in which the recessive alleles are all on one X chromosome (y, w, m) and the dominant alleles are all on the other (B, R, L). [FEMALE Brown Body Red Eyes Large Wings] I mapped genes for three recessive traits – yellow body (y), white eyes (w), and miniature wings (m). First I made heterozygous female strains, in which the recessive alleles are all on one X chromosome (y, w, m) and the dominant alleles are all on the other (B, R, L). Then I crossed these females with males, whose single X chromosome carried the recessive alleles (y, w, m). [MALE Yellow Body White Eyes MIniature Wings] The phenotypes of male hybrids are due to the X chromosome contributed by the mother. The mother's X chromosome also determines the phenotype of all female hybrids – against the "neutral" background of the recessive X inherited from the father. I found, as expected, that half the flies showed the dominant and half the recessive trait. If alleles on the same chromosome were always inherited as an unit, I would have found that all hybrid offspringshow either a purely dominant or purely recessive phenotype. I examined 10,495 flies; in fact, I found that only about two-thirds of the offspring showed the pure dominant or recessive phenotypes. [YELLOW BODY WHITE EYES MINI WING BROWN BODY RED EYES LARGE WING 6972 FLIES] The remaining flies showed mixtures, or recombinations, of dominant and recessive traits. [BROWN BODY RED EYES MINI WING YELLOW BODY WHITE EYES LARGE WING 3454 FLIES] [YELLOW BODY RED EYES LARGE WING BROWN BODY WHITE EYES MINI WING 60 FLIES] [YELLOW BODY RED EYES MINI WING BROWN BODY WHITE EYES LARGE WING 9 FLIES] Each of the mixed phenotypes can be explained by crossing over between the two X chromosomes during egg formation. The largest number of flies were either phenotypically dominant or phenotypically recessive for all three traits. This is whatMendel's laws predict if no crossovers occurred. [BROWN BODY RED EYES LARGE WINGS YELLOW BODY WHITE EYES MINIATURE WINGS NO CROSSOVERS] The relatively high frequency of crossover between eye color and wing size indicates that they are distant on the chromosome. [CROSSOVER BETWEEN EYE COLOR AND WING SIZE] The low frequency recombination between body color and eye color indicates relatively close linkage. [CROSSOVER BETWEEN BODY COLOR AND EYE COLOR] Double crossovers – between body color and eye color and between eye color and wing size – are most rare. [CROSSOVERS BETWEEN BODY AND EYE COLOR AND EYE COLOR AND WING SIZE]
electron micrograph, recessive traits, crossover points, dominant alleles, chromatids, cytologist, Alfred Sturtevant , meiosis, gene mapping, homologous chromosomes, crossing over, double crossover, crossover site, phenotypes, recombinants
- ID: 16280
- Source: DNALC.DNAFTB
DNAFTB Gallery 32: Barbara McClintock presents her work with maize.
DNAFTB Animation 4: Gregor Mendel explains the rules of inheritance.
Mario Capecchi continues his explanation of the technique he uses to control genes in mice.
Herman Muller induces fruit fly mutations. Seymour Benzer works with virus mutants ans proved only one nucleotide change can cause mutation.
DNAFTB Problem 11: Determine gene linkage in fruit flies.
Linkage groups on chromosomes gave clues to where genes are located.
DNAFTB Problem 4: Cross pure-bred pea plants to identify dominant flower color.
DNAFTB Animation 5:Reginald Punnett and William Bateson explain Mendel's ratios.
DNAFTB Problem 10: Perform some fruit fly crosses.
DNAFTB Concept 4: Mendel identifies dominant and recessive genes.