Sequencing mitochondrial DNA, Frederick Sanger
Interviewee: Frederick Sanger. Frederick Sanger talks about the results from sequencing human mitochondrial DNA. (DNAi Location:Manipulation > Techniques > Sorting and sequencing > Interviews > Sequencing mitochondrial DNA)
And one interesting thing we found was that the genetic code was different in the mitochondria from what it was in the rest of the human body, which was quite important. And of course, the actual sequence of the, we got the sequence of the DNA and from that you can work out the sequence of the few proteins that are made. And the sequence of those proteins varies considerably from individual, one individual to another, so one outcome was a method for tracing people's ancestry and, in criminal problems, you can identify differences in DNA of people.
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Frederick Sanger talks about the differences between sequencing proteins and sequencing DNA.
The DNA sequencing method developed by Fred Sanger forms the basis of automated "cycle" sequencing reactions today.
Frederick Sanger describes the use of computers in sequencing.
Frederick Sanger received two Nobel prizes (in the same category), for his work on protein sequencing and DNA sequencing.
Michael Hunkapiller talks about the process of developing the automated sequencing machine.
Early sequencers used four different reactions to determine the placement of each of DNA's four bases - known as A, C, T & G - in the sequence.
Two sequencing techniques were developed independently in the 1970s. The method developed by Fred Sanger used chemically altered "dideoxy" bases to terminate newly synthesized DNA fragments at specific bases (either A, C, T, or G). These fragments are th
A gene is a discrete sequence of DNA nucleotides
Molecular geneticist Douglas Wallace talks about the origin of our mitochondria and the DNA within.
Human mitochondrial DNA is 16,569 base pairs in length.