Sequencing proteins and DNA, Frederick Sanger
Interviewee: Frederick Sanger. Frederick Sanger talks about the differences between sequencing proteins and sequencing DNA. (DNAi Location: Manipulation > Techniques > Sorting and sequencing > Interviews > Sequencing proteins and DNA)
You know, at first we tried to use the methods we'd used for proteins and to some extent these worked. But it was, turned out to be a very different problem because the proteins have twenty components, twenty amino acids, and all different. Whereas the nucleic acids just have the four components, the mononucleotides, and you have to work out sequencing on that. And the methods used for proteins were not generally applicable. For instance they were, the amino acids were all very different chemically, so you could work out methods for separating them. Whereas the nucleic acids only had the four components, which were rather similar and so you knew, had to use quite different methods.
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- ID: 15160
- Source: DNALC.DNAi
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16526. Biography 23: Frederick Sanger (1918-2013)
Frederick Sanger received two Nobel prizes (in the same category), for his work on protein sequencing and DNA sequencing.
15159. Sequencing mitochondrial DNA, Frederick Sanger
Frederick Sanger talks about the results from sequencing human mitochondrial DNA.
15161. Computers and sequencing, Frederick Sanger
Frederick Sanger describes the use of computers in sequencing.
15479. Sanger method of DNA sequencing, 3D animation with narration
The DNA sequencing method developed by Fred Sanger forms the basis of automated "cycle" sequencing reactions today.
15098. Making sequencing automated, Michael Hunkpiller
Michael Hunkapiller talks about the process of developing the automated sequencing machine.
16036. Fred Sanger, 1975
A gene is a discrete sequence of DNA nucleotides
16515. Animation 23: A gene is a discrete sequence of DNA nucleotides.
Fred Sanger outlines DNA sequencing.
15567. DNA sequence
Early sequencers used four different reactions to determine the placement of each of DNA's four bases - known as A, C, T & G - in the sequence.
15922. Early DNA sequencing
Two sequencing techniques were developed independently in the 1970s. The method developed by Fred Sanger used chemically altered "dideoxy" bases to terminate newly synthesized DNA fragments at specific bases (either A, C, T, or G). These fragments are th
16520. Gallery 23: Fred Sanger, late 1950's
Fred Sanger in his lab, late 1950's. He is looking at sequencing results.