Recombinant DNA, David Goeddel
Interviewee: David Goeddel. David Goeddel talks about recombinant DNA.
So taking the synthetic approach, where you synthesize a, a fragment of DNA, let's say coding insulin, now you have this linear piece of DNA, but it\'s not in any form that it can do anything you need to put it in a replicating form. And the most common way to do that is insert it into what's called a bacterial plasmid, which is a small circular piece of DNA that are found in some bacteria. And this can be opened up, essentially with molecular scissors, that allow your synthetic piece to be inserted there and all of it sealed back up, essentially glued back together. Now this circular piece of DNA can easily be put back into the bacteria, where now it is replicated, over and over, in exact copies. And that\'s the recombinant piece, you\'ve taken your piece of DNA and re-combined it into another piece of DNA that can now be replicated in an organism.
bacterial plasmid,molecular scissors,recombinant dna,circular piece,synthetic approach,exact copies,fragment,organism,insulin,bacteria
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
Synthetic human insulin was the first golden molecule of the biotech industry and the direct result of recombinant DNA technology. Currently, millions of diabetics worldwide use synthetic insulin to regulate their blood sugar levels. Synthetic insulin i
Genetic engineering: inserting new DNA into a plasmid vector.
Synthesizing human insulin using recombinant DNA, 3D animation with no audio
Synthetic human insulin was the first golden molecule of the biotech industry and the direct result of recombinant DNA technology. Currently, millions of diabetics worldwide use synthetic insulin to regulate their blood sugar levels.
How was synthetic insulin first made? Although the idea was simple, in practice there were substantial problems. No one had the human DNA sequence for insulin, and there was a moratorium on the production of human recombinant DNA. Two different strateg
Paul Berg discusses the usefulness of recombinant DNA to isolate and study genes.
David Goeddel talks about how another group was trying to make insulin.
Herb Boyer talks about Stanley Cohen's and his interest in plasmids as vectors for DNA.