Gilbert's approach to synthesizing insulin, David Goeddel
Interviewee: David Goeddel. David Goeddel talks about how another group was trying to make insulin. (DNAi Location: Manipulation > Production > Players > David Goeddel > The other strategy)
So the approach at least as far as we were aware, that the Gilbert group was following, was to isolate a natural gene or a copy of a natural gene for human insulin, and then they of course would have the cloning of it in the expression. But the starting point was very different, using natural DNA as composed to synthetic DNA... compared to synthetic DNA.
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Walter Gilbert talks about his group's early success with isolating the rat insulin gene and making recombinant rat insulin.
David Goeddel talks about the other groups working on the insulin problem.
Walter Gilbert's group tried to isolate the human insulin DNA sequence using the rat insulin DNA sequence.
The recombinant DNA moratorium meant Gilbert had to go to England's Porton Down facility to try and isolate human insulin. He only had one chance ...
David Goeddel was one of the first scientists Herb Boyer hired for the insulin project. Here, David Goeddel talks about Genentech's general strategy for synthesizing insulin.
Walter Gilbert talks about close but no cigar.
Walter Gilbert talks about the reasons for making insulin with recombinant DNA.
In the late 1970s, there was a moratorium on recombinant DNA work. Gilbert had to go to England's Porton Down facility to try and isolate human insulin.
Walter Gilbert talks about his feelings regarding the insulin project and science in general.
David Goeddel talks about the strategy Genentech used in making synthetic insulin.