Close but no cigar, Walter Gilbert
Interviewee: Walter Gilbert. Walter Gilbert talks about close but no cigar.
We had this, tried to obtain the human sequence, not the rat sequence, and tried to make it in large enough amounts to be commercially useful. In our case, we failed by a little bit, we had to make twenty milligrams per litre of insulin in order to become commercially useful, we got to nineteen, just under the cut-off line, and the project never became successful. Genentech's case they actually made the human insulin and that human insulin made by a somewhat different procedure, finally went to market. Nowadays of course the only insulin you would get on the market would be synthetically made by these recombinant DNA techniques.
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Walter Gilbert's group tried to isolate the human insulin DNA sequence using the rat insulin DNA sequence.
Walter Gilbert talks about his group's early success with isolating the rat insulin gene and making recombinant rat insulin.
The recombinant DNA moratorium meant Gilbert had to go to England's Porton Down facility to try and isolate human insulin. He only had one chance ...
Walter Gilbert talks about the reasons for making insulin with recombinant DNA.
David Goeddel talks about how another group was trying to make insulin.
In the late 1970s, there was a moratorium on recombinant DNA work. Gilbert had to go to England's Porton Down facility to try and isolate human insulin.
Synthesizing human insulin using recombinant DNA, 3D animation with no audio
Walter Gilbert talks about his feelings regarding the insulin project and science in general.
How Jacob and Monod showed the existence of the inhibitor (what Gilbert calls the repressor).
Jacob and Monod never identified the inhibitor, but Gilbert found it.