Close but no cigar, Walter Gilbert

Interviewee: Walter Gilbert. Walter Gilbert talks about close but no cigar.

We had this, tried to obtain the human sequence, not the rat sequence, and tried to make it in large enough amounts to be commercially useful. In our case, we failed by a little bit, we had to make twenty milligrams per litre of insulin in order to become commercially useful, we got to nineteen, just under the cut-off line, and the project never became successful. Genentech's case they actually made the human insulin and that human insulin made by a somewhat different procedure, finally went to market. Nowadays of course the only insulin you would get on the market would be synthetically made by these recombinant DNA techniques.

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Walter Gilbert's group tried to isolate the human insulin DNA sequence using the rat insulin DNA sequence.

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15256. Producing rat insulin using recombinant DNA, Walter Gilbert

Walter Gilbert talks about his group's early success with isolating the rat insulin gene and making recombinant rat insulin.

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15259. Cloning the human insulin gene, Walter Gilbert

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David Goeddel talks about how another group was trying to make insulin.

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15258. Government restrictions on working with recombinant DNA, Walter Gilbert

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15505. Synthesizing human insulin using recombinant DNA, 3D animation with no audio

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15257. Trying to produce insulin using recombinant DNA, Walter Gilbert

Walter Gilbert talks about his feelings regarding the insulin project and science in general.

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16697. Video 33: Walter Gilbert, clip 4

How Jacob and Monod showed the existence of the inhibitor (what Gilbert calls the repressor).

  • ID: 16697
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16698. Video 33: Walter Gilbert, clip 5

Jacob and Monod never identified the inhibitor, but Gilbert found it.

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