Paul Zamecnik first developed the cell-free extract system, which Marshall Nirenberg adapted to decipher the genetic code. Paul Zamecnik and Mahlon Hoagland also isolated activated tRNA, the "adaptor" that shuttled amino acids to ribsomes for incorporation into new protein chains.
protein chains,free extracts,amino acids,mahlon,genetic code,incorporation,dna,marshall
- ID: 15885
- Source: DNALC.DNAi
Marshall Nirenberg and Heinrich Matthaei used poly-U mRNA in a cell-free system to make a polyphenylalanine protein chain. This showed that UUU must be the code that specifies the amino acid phenylalanine.
Marshall Nirenberg talks about cell-free protein synthesis.
Paul Zamecnik developed a cell-free extract that he and Mahlon Hoagland used to study protein synthesis. They identified tRNA as the adaptor predicted by Francis Crick in his Central Dogma
Francis Crick describes RNA and its role and Paul Zamecnick explains protein synthesis.
Several researchers crack the genetic code.
Marshall Nirenberg talks about RNA can stimulate protein synthesis.
Paul Zamecnik photo
The Central Dogma is the flow of genetic information from DNA, to RNA, to protein.
Three DNA nucleotides form a codon and specifiy amino acids.
Fred Sanger outlines DNA sequencing.