Tedious process of early gene hunting, Mary-Claire King

Interviewee: Mary-Claire King. Tedious process of early gene hunting. (DNAi Location: Timeline > 1990s)

Twenty-five years ago when I began this work this was an extraordinarily tedious process because there were very few such flags, a few dozen. There was also no quick way of evaluating them, so one had to do the evaluation, tedious flag by tedious flag, using procedures that took three weeks from the beginning of the experiment to the end, and then one had one result. And then one went on to one more result. It was incredibly slow going. The invention of PCR made a huge difference to us, because it meant that instead of three weeks we were now down to three hours between the time that we would begin an analysis and could end it. But the most critical component in all of this, next to the families themselves, was the existence of a genetic map, so that we could, for a family like this, identify markers on every single chromosome, all the way up and down chromosome 1, all the way up and down chromosome 2, and so on all the way through chromosome 22 and X and Y.

mary claire king,chromosome 22,chromosome 1,brca1 gene,dnai,twenty five years,interviewee,critical component,invention,flags,1990s,hunting,existence

Related Content

15125. The early stages of the hunt for BRCA1, Mary-Claire King

Mary-Claire King talks about the search for a bit of DNA that would shed light on why some members of a family developed cancer while others did not.

  • ID: 15127
  • Type: video
  • Source: DNAi

15118. What to look for in the hunt for BRCA1, Mary-Claire King

Mary-Claire King talks about her first steps toward finding the gene responsible for certain kinds of inherited breast cancer.

  • ID: 15127
  • Type: video
  • Source: DNAi

15121. Little known in the 1970s about the causes of cancer, Mary-Claire King

Mary-Claire King speaks about how much was yet to be understood about the genetic mechanisms of cancer when she began her hunt for genes associated with breast cancer.

  • ID: 15127
  • Type: video
  • Source: DNAi

15128. Importance of genetic maps, Mary-Claire King

Mary-Claire King talks about the tedious process of hunting for genes in the days before genetic maps (based on thousands of markers) were readily available.

  • ID: 15127
  • Type: video
  • Source: DNAi

15123. Testing for mutations in BRCA1, Mary-Claire King

Mary-Claire King talks about testing for breast cancer.

  • ID: 15127
  • Type: video
  • Source: DNAi

15243. The hunt for BRCA1, Mark Skolnick

Mark Skolnick talks about the hunt for BRCA1.

  • ID: 15127
  • Type: video
  • Source: DNAi

15122. Reaction to Myriad's announcement that BRCA1 had been found, Mary-Claire King

Mary-Claire King recalls her reaction when she heard that the Skolnick team had successfully cloned BRCA1 and made it to the finish line first.

  • ID: 15127
  • Type: video
  • Source: DNAi

15126. Using pedigress in the hunt for BRCA1, Mary-Claire King

Mary-Claire King talks about the value of using the centuries-old tool of family pedigrees to gain insight into patterns of inheritance of genetic disorders.

  • ID: 15127
  • Type: video
  • Source: DNAi

15124. Better treatment for breast cancer, Mary-Claire King

Mary-Claire King reflects on how knowledge gained from the identification of BRCA1 and BRCA2 could lead to improved cancer treatments.

  • ID: 15127
  • Type: video
  • Source: DNAi

16055. Mary-Claire King

MARY-CLAIRE KING (1946- )

  • ID: 15127
  • Type: video
  • Source: DNAi