Sequencing process, Leroy Hood
Interviewee: Leroy Hood. Leroy Hood explains the process of sequencing using an automated sequencing machines. (DNAi Location: Manipulation > Techniques > Sorting & sequencing > Interviews > Inside an automated sequencer)
The individual samples are placed in the wells at the top of the gel, the electrophoretic field is turned on and each of the samples migrate down the gel, small bands travelling more rapidly than large bands. And at the bottom of the gel is a laser that scans across each of the lanes, reading out the color of their four different bands, red, green, yellow and blue, and those represent the four different DNA dyes. The information is recorded in a computer and then the biologist has access to interpreting the data after the run is finished.
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