Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA. Cohen and Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."

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Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA. Cohen and Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby inducing the uptake and expression of a foreign DNA sequence known as "transformation."

e coli bacteria,dna transformation,dna molecule,herbert boyer,stanley cohen,recombinant dna,dna sequence,e coli,plasmid,expression

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16705. Animation 34: Genes can be moved between species.

Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.

  • ID: 15916
  • Type: animation
  • Source: DNALC.DNAFTB

15074. Taking apart plasmid DNA, Stanley Cohen

Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA.

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16721. Biography 34: Herb W. Boyer (1936 - )

Herb Boyer and Stan Cohen "invented" recombinant DNA technology.

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15028. Interest in plasmids, Herbert Boyer

Herb Boyer talks about Stanley Cohen's and his interest in plasmids as vectors for DNA.

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15915. The first recombinant DNA

Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or "recombinant" DNA.

  • ID: 15916
  • Type: animation
  • Source: DNAi

15626. Paul Berg and Stanley Cohen

Herbert Boyer: Former varsity lineman turned biotech bigwig. Expert at cutting DNA before most people knew it could be done. Stanley Cohen: A born tinkerer; figured out the trick of using loops of DNA called plasmids to transform bacterial DNA

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  • Source: DNAi

16720. Biography 34: Stan Norman Cohen (1935 - )

Stan Cohen and Herb Boyer "invented" recombinant DNA technology.

  • ID: 15916
  • Type: animation
  • Source: DNAFTB

15646. Herbert Boyer

Image of Herbert Boyer. From lineman on the varsity football team to co-founder of the first biotech company, Boyer has never lacked imagination, drive, or vision. His and Stanley Cohen's recombinant DNA work paved the way for the biotech revolution.

  • ID: 15916
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  • Source: DNAi

15918. Transformation

DNA transformation is a naturally occuring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more "competent" for transforming foreign DNA. Their calcium chloride method is

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15162. Preparing the DNA for sequencing, Shane Yeager

Shane Yeager, from the Whitehead Institute Center for Genome Research, explains the processes of storing and preparing DNA for sequencing.

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