Using computers to predict how genes within the human genome, Craig Venter
Interviewee: Craig Venter. Craig Venter talks about using computers to predict how genes within the human genome.
Back when we were doing the first test project for sequencing the human genome, the best mathematical tools could only deal with a thousand sequences, and nobody thought you'd ever need anything more than that because that's how the genomes were going to be sequenced, as serial versions of these tiny projects. But all of a sudden we had hundreds of thousands of sequences that we need to coalesce to find out how many human genes there were, what the redundancy and the sequencing was, etc. So we hired some computer scientists and mathematicians, the senior one at the time was Granger Sutton, who built an algorithm that ended up being called the Tiger Assembler. And it was designed to put all these hundreds of thousands of EST CDNA sequences together to guess how many human genes there were.
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Craig Venter, the leader of the private genome effort, talks about the "whole genome shotgun" technique that was used by Celera Genomics to sequence the human genome.
James Watson describes sequencing the human genome using markers and BACs, and Craig Venter explains using cDNA libraries, ESTs, and shotgun sequencing.
For the first draft of the genome sequence, both teams were working to identify the number of human genes. Here, Ewan Birney, a "numbers man" from the public genome project, explains how genes can be recognized and the data from the genome project used.
Craig Venter speaks about the public sector's reaction to his plans to sequence the genome at a private company, Celera Genomics.
Craig Venter, the leader of the private genome effort at Celera Genomics, talks about the sources of the DNA used in their sequence.
Ewan Birney talks about finding genes.
Craig Venter, leader of the private effort at Celera Genomics, speaks about his company's reliance on the public data for reassembly of the Celera sequence.
The leaders of the private and public genome projects, Craig Venter and Francis Collins.
Commentators on the genome sequence (Human Genome Project). Top: William Clinton, Ewan Birney, John Sulston. Bottom: Jim Watson, Craig Venter.
Shotgun sequencing and dealing with repeat sections.