Whole genome shotgun, Craig Venter
Interviewee: Craig Venter. Craig Venter, the leader of the private genome effort, talks about the "whole genome shotgun" technique that was used by Celera Genomics to sequence the human genome. (DNAi Location: Genome > The project > Players > Private project > Whole genome shotgun)
The key step is obviously making a DNA sequencing library, and in the case of Celera that was the most critical step. Because with whole genome shotgun sequencing, if you don't have DNA libraries â€“ these clones that are derived from breaking the genome down into smaller pieces that really represent the genome in a random fashion â€“ it doesn't matter how good the rest of the techniques are of sequencing, mathematics of assembly. It doesn't matter how big your computer is, you can't regenerate the genome from it. We made several different sizes of DNA, it's another critical part in the mathematics of the assembly. So we had pieces that were two thousand letters long, pieces that were ten thousand, pieces that were fifty thousand â€“ a new cloning technique developed by Rob Holt and Ham Smith at Celera â€“ and pieces that were a hundred and fifty thousand letters long.
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James Watson describes sequencing the human genome using markers and BACs, and Craig Venter explains using cDNA libraries, ESTs, and shotgun sequencing.
Craig Venter, the leader of the private genome effort at Celera Genomics, talks about the sources of the DNA used in their sequence.
Eric Lander talks about analysis in public and private Human Genome Projects.
Craig Venter, leader of the private effort at Celera Genomics, speaks about his company's reliance on the public data for reassembly of the Celera sequence.
Craig Venter, the leader of the private genome effort at Celera Genomics, talks about the differences between the public and private approaches.
The leaders of the private and public genome projects, Craig Venter and Francis Collins.
15477. The public Human Genome Project: mapping the genome, sequencing, and reassembly. 3D animation.
The public Human Genome Project: mapping the genome, sequencing, and reassembly.
Craig Venter talks about working with repeats.
Shotgun sequencing and dealing with repeat sections.