The molecular biology of the lac operon, Fraois Jacob
Interviewee: Fran�ois Jacob. Fran�ois Jacob talks about the relationship between DNA, ribosomes, and protein production. (DNAi Location: Code > Copying the code > Players > Fran�ois Jacob > What drives protein production?)
The idea was that when you had the gene galactosidase, the enzyme synthesis starts immediately. Now if we were right and if there was no stable ribosome, if you can remove the gene then it should stop. And we could remove the gene because we had done in Paris an experiment where we loaded the donor chromosome with P32, radioactive P32, then let it go into the recipient, then put it in the cold and let it decay and see whether once the chromosome has been decaying it still makes enzyme on there, and the answer was no. That is as soon as the gene is destroyed by P32 decay, then the capacity to produce the enzyme is stopped.
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François Jacob explains why Jacques Monod chose to work with the lactose system.
François Jacob talks about how enzyme production was viewed at the time they were beginning their experiments.
François Jacob talks about bacterial mutants that could not metabolize lactose. Using these mutants, Jacob and Monod figured out how protein production is controlled.
Jacques Monod and Francois Jacob work with how bacteria breaks large sugars into smaller pieces.
François Jacob, Sydney Brenner and Matt Meselson worked on the role of mRNA. An on/off switch involving mRNA seemed a logical control point for protein production.
François Jacob talks about Genes control the structure of proteins
The first model for the control of protein production was the lac operon. This system of feedback and negative regulation is used by bacteria, however, the general principles also apply to higher organisms. Another method of protein regulation involves
Francois Jacob and Jacques Monod figured out how bacteria controlled the production of an enzyme called beta-galactosidase. This system of feedback and negative regulation became the lac operon and was the first model for the control of protein productio
François Jacob talks about how he and Jacques Monod named messenger RNA.
Francois Jacob and Jacques Monod were the first to discover how genes were turned on and off.