Discovering the lac operon, Walter Gilbert
Interviewee: Walter Gilbert. Walter Gilbert talks about identifying the lac operon repressor. (DNAi Location: Code > Controlling the code > Players > Walter Gilbert > The lac operon repressor)
I and Benno Muller-Hill, who was at that time a post-doc with me, started to work on that problem involving the repressor for the lactose operon, and on a set of genes involving the metabolism of the sugar lactose. We worked on it because we knew it was a fundamental problem in biology, and the problem that we were attacking was what is the nature of the product made by one gene that turns off a second one. The answer in those days that we came to was the product is a protein that goes and binds to DNA and prevents a second gene from functioning. The small molecule of sugar coming into the cell, inactivates that protein, makes it release its hold on DNA and then the genes begin to function. There are only ten molecules of that protein in a bacterial cell, it was extremely hard to identify, and we did a set of experiments in the middle Sixties that actually enabled us to identify that protein, it was the first control protein identified.
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Walter Gilbert describes how they did the experiment to isolate the lac operon repressor.
François Jacob talks about bacterial mutants that could not metabolize lactose. Using these mutants, Jacob and Monod figured out how protein production is controlled.
François Jacob talks about Genes control the structure of proteins
Jacques Monod and Francois Jacob work with how bacteria breaks large sugars into smaller pieces.
Small image of Francois Jacob's lac operon drawing.
François Jacob talks about how enzyme production was viewed at the time they were beginning their experiments.
Learn more about the lac operon system.
Francois Jacob and Jacques Monod figured out how bacteria controlled the production of an enzyme called beta-galactosidase. This system of feedback and negative regulation became the lac operon and was the first model for the control of protein productio
François Jacob explains why Jacques Monod chose to work with the lactose system.
Organisms can regulate gene expression.