Cohesive (sticky) ends and their significance in genetic engineering, Paul Berg
Interviewee: Paul Berg. Cohesive (sticky) ends and their significance in genetic engineering.
Cohesive end in this particular context means that if you take two DNAs that have single strands protruding from their ends, and if these single strands are able to pair with each other by the same rules that DNA strands are held together, then these two molecules could come together. And what Janet showed was that if two DNAs were cut with this particular enzyme, called Eco R-1, then they could be joined and fused together to make recombinant DNAs. And that was a hugely important discovery, because it bypassed the need of the complicated procedures that we had developed in order to bring two molecules together.
recombinant dnas,dna strands,berg california,sticky ends,recombinant dna,paul berg,ecor1,interviewee,genetic engineering,recombination,molecules,manipulation,discovery
Paul Berg speaks about his student Janet Mertz's experiment to make the first recombinant DNA molecule.
Paul Berg talks about possible dangers of recombinant DNA.
Paul Berg discusses the usefulness of recombinant DNA to isolate and study genes.
Genetic engineering: inserting new DNA into a plasmid vector.
Image of Paul Berg, Brooklyn-bred chemistry whiz. Known for his pioneering work in recombinant DNA, which won him a Nobel Prize in 1980.
Paul Berg recollects his reaction to his colleague Bob Pollack's opposition to experimentation with recombinant DNA.
Paul Berg's student, Janet Mertz, planned an experiment that would recombine DNA from a monkey virus with DNA from a bacterium that lives in the human gut. Berg describes colleague Bob Pollack's outrage at this.
Paul Berg speaks about Herbert Boyer's research into the process by which an organism, such as a bacterium, can recognize and destroy foreign DNA.
Paul Berg talks about why experiments with recombinant DNA set off a firestorm of controversy, including a moratorium on further experimentation with rDNA.
Asilomar meeting. February 1975. (L to R) Maxine Singer, Norton Zinder, Sydney Brenner, Paul Berg.