Cold Spring Harbor Laboratory’s DNA Learning Center presented this course as a service to help engage teachers and students in China during the coronavirus school closures.
The DNA restriction analysis experiment demonstrates that DNA can be precisely manipulated and that it behaves as predicted by the Watson-Crick structure. Students use restriction enzymes, the scissors of molecular biologists, to cut DNA from the bacteriophage lambda. The resulting DNA fragments are analyzed by agarose gel electrophoresis.
58 minutes, 27 seconds
Posted: February 20, 2020
dna ligase,dna fragments,restriction enzymes,genetic engineering,bacteria,sequences,discovery, dna gel electrophoresis,dna fragments,dna manipulation,electrophoresis gel,gel matrix,gravity,electricity,Phil Sharp, Joe Sambrook, Bill Sugden
- ID: 17055
- Source: DNALC
In the early days of DNA manipulation, DNA fragments were laboriously separated by gravity. In the 1970s, the powerful tool of DNA gel electrophoresis was developed.
Rich Roberts and Phil Sharp explain restriction enzymes, electrophoresis, and split genes.
The discovery of enzymes that could cut and paste DNA made genetic engineering possible.
Developing the experimental technologies used to study DNA fragments: agarose gel electrophoresis and restriction enzymes.
Phil Sharp and Richard Roberts shared the 1993 Nobel Prize for the discovery of the split gene theory.
Fred Sanger outlines DNA sequencing.
Gel photo of PCR amplification to detect GMO or transgenes in food.
Leroy Hood explains the process of sequencing using an automated sequencing machines.
New York high school students interview Dr. Scott Lowe of Memorial Sloan-Kettering Cancer Center about using restriction enzyme analysis in cancer research, then perform the experiment.
An animation introducing the concept of a DNA barcode, how it works, and what type of research questions DNA barcoding can answer.