New York Stories: Restriction Enzyme Analysis
This is the fourth in a series of mini-documentaries about past or current work of notable scientists based at New York institutions. The vodcasts are produced by New York high school students with the help of the DNALC.
DNA; gene; genetics; New York; high school; gel electrophoresis, recombinant, restriction, enzyme, MSKCC, Sloan-Kettering, Lowe, Trinity, cancer, cut site
- ID: 16995
- Source: DNALC
- Download: MP4 Video
New York high school students perform the Alu polymorphism lab then interview Prof. Larry Kobilinsky at John Jay College of Criminal Justice about DNA fingerprinting.
Rich Roberts and Phil Sharp explain restriction enzymes, electrophoresis, and split genes.
Harold Varmus, President and Chief Executive Officer of Memorial Sloan-Kettering Cancer Center.
Genetic engineering: inserting new DNA into a plasmid vector.
Use green fluorescent protein to tag expression of genes.
Conventional cancer drugs are cellular poisons that block replication or some other aspect of cell growth. These drugs affect all cells – healthy or cancerous.
Experiment with gene knock outs.
TeaBOL is a DNA barcoding project where New York City students explored the genetic biodiversity of different tea brands.
Harold Varmus and Mike Bishop worked out how retroviruses transform normal cells to cancerous ones.