Cutting and pasting DNA
The discovery of enzymes that could cut and paste DNA made genetic engineering possible. Restriction enzymes, found naturally in bacteria, can be used to cut DNA fragments at specific sequences, while another enzyme, DNA ligase, can attach or rejoin DNA fragments with complementary ends.
dna ligase,dna fragments,restriction enzymes,genetic engineering,bacteria,sequences,discovery
- ID: 15917
- Source: DNALC.DNAi
Genetic engineering: inserting new DNA into a plasmid vector.
Stanley Cohen and Herbert Boyer transform bacteria with a recombinant plasmid, and Doug Hanahan studies induced transformation.
Rich Roberts and Phil Sharp explain restriction enzymes, electrophoresis, and split genes.
Eric Lander talks about building on scientific discovery.
DNA with "sticky ends" can be rejoined and ligated together.
Mark Skolnick talks about using RFLPs or SNPs to study patterns in families
A representation of a restriction enzyme cutting DNA.
Once scientists could cut DNA, they still needed a way to paste DNA strands together at will.
Restriction enzymes can bind to and cut DNA at specific sites.