Making GeneChips® at Affymetrix

A technician uses a computer guiding system to align the filter mask in perfect register with the quartz wafer. The filter mask will block light to selected regions of the wafer. More nucleotides can be added to the exposed regions.

quartz wafer,filter mask

  • ID: 15664
  • Source: DNALC.DNAi

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15666. Making GeneChips® at Affymetrix

The quartz wafer is in the holding position on the DNA synthesizer. The wafer is moved to a vertical reaction vessel for the process of DNA chain elongation.

  • ID: 15666
  • Source: DNALC.DNAi

15662. Making GeneChips™ at Affymetrix

Here you can see a technician shuttling a quartz wafer (containing 50-400 GeneChips™) between alternating photolithography and DNA synthesis steps.

  • ID: 15662
  • Source: DNAi

15665. Making GeneChips™ at Affymetrix

A technician loads a quartz wafer into the DNA synthesizer, where nucleotides will be added to selected sequences to build probes.

  • ID: 15665
  • Source: DNAi

15667. Making GeneChips® at Affymetrix

Here a technician aligns a quartz wafer, from which 50-400 GeneChips are prepared for mounting.

  • ID: 15667
  • Source: DNALC.DNAi

15663. Making GeneChips™ at Affymetrix

A technician prepares to load a filter mask into the photolithography camera. These filters allow the technician to build customized chains of DNA. The blue boxes on the right store 50-100 filters needed to produce a single type of GeneChip™.

  • ID: 15663
  • Source: DNAi

15661. Making GeneChips™ at Affymetrix

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  • ID: 15661
  • Source: DNAi

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  • ID: 16725
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