Two sequencing techniques were developed independently in the 1970s. The method developed by Fred Sanger used chemically altered "dideoxy" bases to terminate newly synthesized DNA fragments at specific bases (either A, C, T, or G). These fragments are then size-separated, and the DNA sequence can be read.
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Fred Sanger,dna fragments,dna sequencing,dna sequence,1970s, Sanger Sequencing, amplification, dideoxy, chain termination, dideoxynucleotides, nucleotides
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