Tom Sargent did some of the first differential gene expression studies using cDNA subtraction.
Sargent's gene library and comparisons with today's gene chips.
Figure from Sargent and Dawid's differential expression experiment in frog embryos.
Part I: Theories on how organisms end up with differentiated cells.
Part II: Theories on how organisms end up with differentiated cells.
Molecules that regulate development are similar in different organisms.
Why use other animal models to study development?
The cell as a micro-computer that can interpret signals for different responses.
Igor Dawid and Thomas Sargent explain how they developed subtractive mRNA hybrization to find genes expressed by different cell types. Pat Brown and Steve Fodor show how genomes can be screened with DNA arrays and GeneChips™
RNA was the first genetic molecule.