Using argobacterium to genetically engineer plants, Robert Horsch
Interviewee: Robert Horsch. Robert Horsch explains the mechanism by which agrobacterium delivers its DNA "parcel." (DNAi Location: Manipulation > Techniques > Transferring & storing > Interviews > Using agrobacterium)
As it turns out the agrobacterium doesn't know or care what's in the package of DNA that it transfers into the plant cell genome. And so we can go in with recombinant techniques and cut out the genes that make the gall, cut out the genes that make the opines, and put in any genes that you can engineer. Then the bacterium will transfer it without knowing any difference, and we therefore have a way to engineer a living plant cell using this natural system.
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Robert Horsch talks about the parasitic nature of agrobacterium and the effect it has on the host plant.
Robert Horsch talks about the gene gun: a physical method of delivering genes into plant cells.
Robert Horsch compares the random power of a gene gun with the natural genetic engineering abilities of agrobacterium.
Robert Horsch talks about agrobacterium as a ready-made delivery system for getting foreign DNA into plants.
Image of Dr. Robert Horsch
Joshua Lederberg worked with bacterial genetics while Alfred Hershey showed that DNA is responsible for the reproduction of new viruses in a cell.
Paul Berg discusses the usefulness of recombinant DNA to isolate and study genes.
Mitochondria contain a circular genome molecule, tightly packed with genes.
Paul Berg talks about why experiments with recombinant DNA set off a firestorm of controversy, including a moratorium on further experimentation with rDNA.
The recombinant revolution allowed DNA to be moved between species.