Using argobacterium to genetically engineer plants, Robert Horsch
Interviewee: Robert Horsch. Robert Horsch explains the mechanism by which agrobacterium delivers its DNA "parcel." (DNAi Location: Manipulation > Techniques > Transferring & storing > Interviews > Using agrobacterium)
As it turns out the agrobacterium doesn't know or care what's in the package of DNA that it transfers into the plant cell genome. And so we can go in with recombinant techniques and cut out the genes that make the gall, cut out the genes that make the opines, and put in any genes that you can engineer. Then the bacterium will transfer it without knowing any difference, and we therefore have a way to engineer a living plant cell using this natural system.
recombinant dna technology,robert horsch,nooks and crannies,what is agrobacterium,bacterial colonies,manipulation techniques,plant cells,dust particles,dnai,carbon source,host plant,opines,interviewee,weather,genes,nitrogen,compounds,bacteria,segment,soil
- ID: 15264
- Source: DNALC.DNAi
- Download: MPEG 4 Video Theora Video
15263. What is agrobacterium?, Robert Horsch
Robert Horsch talks about the parasitic nature of agrobacterium and the effect it has on the host plant.
15265. Plant transformation using the gene gun, Robert Horsch
Robert Horsch talks about the gene gun: a physical method of delivering genes into plant cells.
15267. Different methods of genetically engineering plants - Robert Horsch
Robert Horsch compares the random power of a gene gun with the natural genetic engineering abilities of agrobacterium.
15266. Genetically engineering plants using agrobacterium, Robert Horsch
Robert Horsch talks about agrobacterium as a ready-made delivery system for getting foreign DNA into plants.
15658. Robert Horsch
Image of Dr. Robert Horsch
16395. Animation18: Bacteria and viruses have DNA too.
Joshua Lederberg worked with bacterial genetics while Alfred Hershey showed that DNA is responsible for the reproduction of new viruses in a cell.
15024. The origin and utility of recombinant DNA, Paul Berg
Paul Berg discusses the usefulness of recombinant DNA to isolate and study genes.
16646. Concept 30: Higher cells incorporate an ancient chromosome.
Mitochondria contain a circular genome molecule, tightly packed with genes.
15023. The experiment that galvanized the scientific community, Paul Berg
Paul Berg talks about why experiments with recombinant DNA set off a firestorm of controversy, including a moratorium on further experimentation with rDNA.
15639. Recombinant revolution (human and frog)
The recombinant revolution allowed DNA to be moved between species.