Genome sequencing: missing a stage, John Sulston
Interviewee: John Sulston. John Sulston, a key figure in the public genome project, speaks about the difficulties posed by missing a step in the sequencing process. (DNAi Location: Genome >The project > Players > Private project > Genome shotgun: missing a stage)
The Celera approach was to miss out this intermediate stage, they did a single shotgun going straight from the genome down to small pieces. So when they started to reassemble, of course you can do a lot, it goes quite well for large parts of the genome, but when you come to places where there's severe confusion between a sequence here and a sequence over here, then you've got something to deal with.
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Nobel Laureate John Sulston, a key figure in the UK sequencing effort, talks about breaking DNA apart so that the sequence can be reassembled.
Commentators on the genome sequence (Human Genome Project). Top: William Clinton, Ewan Birney, John Sulston. Bottom: Jim Watson, Craig Venter.
Shotgun sequencing and dealing with repeat sections.
15477. The public Human Genome Project: mapping the genome, sequencing, and reassembly. 3D animation.
The public Human Genome Project: mapping the genome, sequencing, and reassembly.
Nobel Laureate John Sulston, former director of the Wellcome Trust Sanger Institute, talks about the competition within the public sequencing effort, and the distraction of the private.
Eric Lander talks about whole genome shotgun.
Craig Venter, the leader of the private genome effort, talks about the "whole genome shotgun" technique that was used by Celera Genomics to sequence the human genome.
John Sulston talks about response of the media.
Nobel Laureate John Sulston reflects on the Human Genome Project from an evolutionary perspective.
Craig Venter, leader of the private effort at Celera Genomics, speaks about his company's reliance on the public data for reassembly of the Celera sequence.