Live Hands-On high school labs will be taught in two sessions of 2 hours each. Student kits will contain consumable materials to perform a DNA isolation* (35 students max per class.) DNA samples will be batched by the teacher, and returned to the DNALC in person or by mail (at their own cost) for processing.
Session I (2 hours): Wet Lab - Guided by a DNALC instructor, students use kit materials to perform DNA isolations from their own*, or other animal / plant cells. DNA samples will be batched by the teacher, and returned to the DNALC in person or by mail (at their own cost) for processing.
Session II (2 hours, 2 weeks after Session I): Analysis - DNALC instructor will demonstrate DNA processing techniques, including but not limited to: gel electrophoresis, PCR and DNA sequencing. Students will view their results as a class and conduct analysis of results using appropriate bioinformatics tools. Lab results will be uploaded to DNALC websites for further analysis and lab reports.
*Please be aware that our current protocol for DNA isolation from human cells requires collection of epithelial cells from the inside lining of the cheek. For safety reasons, we recommend that students perform this part of the lab from home. We are in the process of developing an alternate protocol for isolation from hair follicles, which will replace cheek cells for students who are in school full time.