DNA Barcoding Part II: PCR and Gel Electrophoresis

Date: 4/8/2020
Time: 1:00 PM
Duration: 60 minutes
Presenter(s): Allison Mayle, DNALC NYC
Audience: High school and college students and educators
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Activity description:

DNA Barcoding, Part 2 (of 3)

DNA barcoding allows for the identification of an organism through analysis of a short region of DNA within its genome, similar to how the pattern of bars in a universal product code (UPC) identifies each consumer product. Follow Dr. Allison Mayle through this three-part series as she introduces you to the wet lab procedures behind isolating, amplifying, and sequencing a barcode region of an organism’s DNA to identify the species!

In this DNALC Live session, you will:

• Gain an understanding of how Polymerase Chain Reaction (PCR) is used to make many copies of a specific region of DNA

• Explore how gel electrophoresis is used to separate and visualize fragments of DNA

• Amplify and observe the DNA barcode region from the processed samples in Part I