DNALC LIVE
DNA Barcoding Part II: PCR and Gel Electrophoresis
Activity description:
DNA Barcoding, Part 2 (of 3)
DNA barcoding allows for the identification of an organism through analysis of a short region of DNA within its genome, similar to how the pattern of bars in a universal product code (UPC) identifies each consumer product. Follow Dr. Allison Mayle through this three-part series as she introduces you to the wet lab procedures behind isolating, amplifying, and sequencing a barcode region of an organism’s DNA to identify the species!
In this DNALC Live session, you will:
• Gain an understanding of how Polymerase Chain Reaction (PCR) is used to make many copies of a specific region of DNA
• Explore how gel electrophoresis is used to separate and visualize fragments of DNA
• Amplify and observe the DNA barcode region from the processed samples in Part I