Bacterial Transformation and DNA Restriction Analysis
Teacher Professional Development in Genetics and Biotechnology
Election Day: November 2, 2021, 8:30 a.m.–2:30 p.m. EDT
Regeneron DNA Learning Center, Sleepy Hollow, NY
This experiment illustrates the direct link between an organism's genetic complement (genotype) and its observable characteristics (phenotype), and demonstrates how genetic engineering is used to produce human proteins such as insulin. In this lab you will introduce genes for antibiotic resistance and fluorescence into the bacterium E. coli. Following overnight incubation, transformed bacteria can be compared to control bacteria for their ability to grow in the presence of ampicillin and glow when exposed to UV light.
DNA Restriction Analysis
This experiment demonstrates that DNA can be precisely manipulated and that it behaves as predicted by the Watson-Crick structure. You will use restriction enzymes, the scissors of molecular biologists, to cut bacteriophage lambda DNA. The resulting DNA fragments will then be analyzed by agarose gel electrophoresis. Through comparison of DNA digest patterns and restriction maps, you will be able to identify a “mystery” enzyme.
These experiments directly correlate with lab requirements for Living Environment and AP Biology courses and align with the New York State Science Learning Standards, with an emphasis on analyzing and interpreting data, planning and carrying out investigations and the structure and function relationship. Guided by an experienced DNALC educator, training will focus on lab skills, conceptual connections, and classroom implementation.
*Participants will receive a Certificate of Completion and CTLE hours.
*Participants must provide proof that they are fully vaccinated. You will receive a link by email to upload this documentation directly to the DNALC. Masks are required at the DNALC.